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KMID : 1025520040460060967
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Journal of Animal Science and Technology
2004 Volume.46 No. 6 p.967 ~ p.974
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Effect of Isomers of Conjugated Linoleic Acid on Porcine Preadipocyte Differentiation
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Moon H.-S.
Chung Chung-Soo
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Abstract
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The current study was undertaken to determine the effect of various conjugated linoleic acid (CLA) isomers on differentiation of pig preadipocyte during culture. Preadipocyte(stroma-vascular cell) was isolated from the backfat of newborn pigs and cultured to differentiate into mature fat cell. Different doses of CLA isomers were treated to the culture media at different times. Cell differentiation was determined by measuring the glycerol- 3-phosphate dehydrogenase activity of the cultured preadipocytes. Twenty and fifty μM of trans10-cis12 isomer of CLA inhibited differentiation of pig preadipocyte whereas cis9-cis11 isomer stimulated the differentiation. Both cis9-trans11 and trans9-trans11 isomers showed no effect. Effect of CLA isomer was more evident at the early stage of culture(day 0-8), than the late stage(day 8-14). These results suggest that each CLA isomer has different effect on pig preadipocyte differentiation.
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KEYWORD
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CLA, Pig, Preadipocyte, Differentiation
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